Defensive publication

ABSTRACT

THE RATE AND EFFICACY OF EXTRACTION OF MILK CLOTTING ENZYMES FROM ANIMAL TISSUE IS IMPROVED BY CONTACTING THE ANIMAL TISSUE IN AN EXTRACTION ZONE WITH A BRINE SOLUTION CONTAINING ABOUT 2 TO 10 WEIGHT PERCENT SALT, REMOVING AN EFFLUENT CONTAINING EXTRACED MILK ENZYME FROM THE EXTRACTION ZONE, SEPARATING THE MILK CLOTTING ENZYMES FROM THE REMOVED EFFLUENT BY PASSING IT THROUGH A HIGH PRESSURE REVERSE OSMOSIS ZONE WHICH INCLUDES A MEMBRANE CAPABLE OF PASSING SALT AND WATER WHILE RETAINING THE MILK CLOTTING ENZYMES, REMOVING THE CONCENTRATE CONTAINING THE MILK CLOTTING ENZYMES FROM THE REVERSE OSMOSIS ZONE, REMOVING THE PERMEATE CONTAINING SALT AND WATER FROM THE REVERSE OSMOSIS ZONE, ADJUSTING THE SALT CONCENTRATION IN THE PERMEATE, AND RECYCLING THE THUS-ADJUSTED PERMEATE TO THE EXTRACTION ZONE.

UNITED STATES PATENT OFFICE Published at the request of the applicant orowner in accordance with the Notice of Dec.';.16, 1969, 869 O.G. 687.The abstracts of Defensive Publication applications are identified bydistinctly numbered series and are arranged chronologically. The headingof each abstract indicates the number of pages of specification,including claims and sheets of drawings contained in the application asoriginally filed. The files of these applications are available to thepublic for inspection and reproduction may be purchased for 30 cents asheet.

Defensive Publication applications have not been examined as to themerits of alleged invention. The Patent Ofiice makes no assertion as tothe novelty of the disclosed subject matter.

PUBLISHED AUG. 1, 1972 T901 012 CONCENTRATION OF ANIMAL-DERIVED MILKCLOTTHNG ENZYMES BY REVERSE OSMOSIS John H. Nelson, Waukesha, Wis.,assignor to Dairyland Food Laboratories, Inc., Waukesha, Wis.

Continuation of application Ser. No. 842,694, July 17,

1969. This application Aug. 31, 1971, Ser. No. 176,746

Int. Cl. (307g 7/02, 7/026 US. Cl. 195-66 R No Drawing. 12 PagesSpecification The rate and efficacy of extraction of milk clottingenzymes from animal tissue is improved by contacting the animal tissuein an extraction zone with a brine solution containing about 2 to 10weight percent salt, removing an effluent containing extracted milkenzyme from the extraction zone, separating the milk clotting enzymesfrom the removed efliuent by passing it through a high pressure reverseosmosis zone which includes a membrane capable of passing salt and Waterwhile retaining the milk clotting enzymes, removing the concentratecontaining the milk clotting enzymes from the reverse osmosis zone,removing the permeate containing salt and water from the reverse osmosiszone, adjusting the salt concentration in the permeate, and recyclingthe thus-adjusted permeate to the extraction zone.

